English biologist and cell cycle expert who worked at the Roslin Institute in Scotland on the development of the process to create the first clones from adult cells.
It was his research on cell cycles that opened the way to the cloning of mammals from differentiated cells.
Research Interests : Animal cell and developmental biology and biotechnology; including the molecular and cellular basis of developmental competence during oocyte maturation and early development (with particular reference to the role of the cell cycle and chromatin structure in development and differentiation); embryo production and manipulation techniques; the use of transgenic animals for human therapeutics.
The application of modern reproductive technologies to the livestock industry provides methods (both in vivo and in vitro) for increasing the production of embryos of high genetic merit, for selection or production of embryos of defined sex and for the preservation of both embryos and sperm. More recently the development of Nuclear Transfer Techniques has allowed the production of multiple genomic copies of individuals utilizing both embryonic blastomeres and somatic cells as donors of genetic material. The use of Nuclear Transfer has also provided a route for the precise genetic modification of farm animal species, thus providing a route for the study of gene function, the production of biopharmaceutical proteins, modification of production traits or disease susceptibility and a method for the preservation of genetic diversity. At the present time the frequency of development to term of embryos reconstructed by Nuclear Transfer is extremely low, approximately 2% of successful reconstructions. In addition the frequency of precise genetic modification in cultured somatic cells is also very low. The aims of this research group are threefold; firstly to study early developmental events in embryos and embryos reconstructed by nuclear transfer in order to understand the interaction of a range of genetic and epigenetic factors on development, in particular to study early differentiation events. Secondly to study basic mechanisms of homologous recombination in somatic cell populations in order to improve the frequency of success of precise genetic modification. Thirdly to combine these techniques for the production of transgenic animals for the study of gene function in differentiation, development and disease, for the production of human therapeutic proteins or for the study of differentiation for cell based therapies.
Selected Publications : McCreath, K.J., Howcroft, J., Campbell, K.H.S., Colman, A., Schnieke, A.E. and Kind, A.J. (2000). Homologous recombination in ovine somatic cells enables the production of gene targeted sheep by nuclear transfer. Nature 405:1066-90
Polajaeva, I, Chen, S-H. Vaught, T. Page, R., Mullins, J. Colman, A. & Campbell, K.H.S. (2000). Cloned Piglets from Adult Somatic Cells. Nature 407: 86-90
Keith H. S. Campbell. (1999). Nuclear Equivalence, Nuclear Transfer and the Cell Cycle. Cloning, 1: 3-15
Keith H. S. Campbell. (1999). Cloning farm Animal Species. Seminars in Cell and Developmental Biology. 10:245-252.
Campbell K.H.S. & Wilmut, I. (1998) Nuclear Transfer. In Animal Breeding: Technology for the 21st Century. Ed: A.J. Clark (Harwood Academic Publishers). P47-62
Campbell, K. H. S. (1998). Cloning Dolly:Implications for Human Medicine. In ‘Fertility and Reproductive Medicine’ (Eds: R. D. Kempers, J. Cohen, A. F. Haney, and J. B. Younger) pp3-11.(Excerpta Medica: International Congress series 1183).
Campbell, K. H. S and Wilmut, I. (1997). Totipotency or Multipotentiality of Cultured Cells:Applications and Progress. Therio 47, 72.
Wilmut, I., Schnieke, A.E., McWhir, J., Kind, A.J., & Campbell, K.H. (1997). Viable offspring derived from fetal and adult mammalian cells [see comments] [published erratum appears in Nature 1997 Mar 13;386(6621):200]. Nature, 385, 810-813.
Schnieke, A.E., Kind, A.J., Ritchie, W.A., Mycock, K., Scott, A.R., Ritchie, M., Wilmut, I., Colman, A., & Campbell, K.H. (1997). Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278, 2130-2133.